Título:
A peculiar cell cycle arrest at g2/m stage during the stationary phase of growth in the wine yeast Hanseniaspora vineae
Otros títulos:
Coordinador:
Director:
Compilador:
Autor:
Schwarz, Luisa Vivian
Valera, María José
Longaray Delamare, Ana Paula
Carrau, Francisco
Echeverrigaray, Sergio
Valera, María José
Longaray Delamare, Ana Paula
Carrau, Francisco
Echeverrigaray, Sergio
Tutor:
Tipo de documento:
Artículo
Editor:
Palabras clave:
LEVADURAS
HANSENIASPORA VINEAE
CICLO CELULAR
VIABILIDAD CELULAR
HANSENIASPORA VINEAE
CICLO CELULAR
VIABILIDAD CELULAR
Descriptores:
Año de publicación:
2022
Contenido:
Resumen:
Yeasts of the genus Hanseniaspora gained notoriety in the last years due to their contribution to wine quality, and
their loss of several genes, mainly related to DNA repair and cell cycle processes. Based on genomic data from
many members of this genus, they have been classified in two well defined clades: the “faster-evolving linage”
(FEL) and the “slower-evolving lineage” (SEL). In this context, we had detected that H. vineae exhibited a rapid
loss of cell viability in some conditions during the stationary phase compared to H. uvarum and S. cerevisiae. The
present work aimed to evaluate the viability and cell cycle progression of representatives of Hanseniaspora
species along their growth in an aerobic and discontinuous system. Cell growth, viability and DNA content were
determined by turbidity, Trypan Blue staining, and flow cytometry, respectively. Results showed that H. uvarum
and H. opuntiae (representing FEL group), and H. osmophila (SEL group) exhibited a typical G1/G0 (1C DNA)
arrest during the stationary phase, as S. cerevisiae. Conversely, the three strains studied here of H. vineae (SEL
group) arrested at G2/M stages of cell cycle (2C DNA), and lost viability rapidly when enter the stationary phase.
These results showed that H. vineae have a unique cell cycle behavior that will contribute as a new eukaryotic
model for future studies of genetic determinants of yeast cell cycle control and progression.
Descripción:
metadata.articulos.dc.description.uri:
Editorial:
Elsevier
EN:
Current Research in Microbial Sciences v.3, 2022. -- e100129
Financiadores:
Citación:
Schwarz, L, Valera, M, Longaray Delamare, A, [y otros autores]. "A peculiar cell cycle arrest at g2/m stage during the stationary phase of growth in the wine yeast Hanseniaspora vineae". Current Research in Microbial Sciences. [en línea] 2022, 3: e100129, doi: https://doi.org/10.1016/j.crmicr.2022.100129
Citación:
metadata.articulos.cc.license.name:
ISBN:
e-ISBN:
ISSN:
ISMN:
Otros identificadores:
Cobertura geográfica:
Cobertura temporal:
Departamento académico:
Grupo de investigación:
Licencia:
Licencia Creative Commons Atribución (CC - By 4.0)
Colecciones:
| Ficheros | Descripción | Tamaño | Formato | ||
|---|---|---|---|---|---|
| AA Schwarz, L. V. et al. Current Research in Microbial Sciences v.3, 2022. -- e100129.pdf | — | 1.11 MB | Adobe PDF |