Título:
A Comparative Analysis of the Protein Cargo of Extracellular Vesicles from Helminth Parasites
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Autor:
Ancarola, María Eugenia
Maldonado, Lucas L.
García, Lucía C. A.
Franchini, Gisela R.
Mourglia-Ettlin, Gustavo
Kamenetzky, Laura
Cucher, Marcela A.
Maldonado, Lucas L.
García, Lucía C. A.
Franchini, Gisela R.
Mourglia-Ettlin, Gustavo
Kamenetzky, Laura
Cucher, Marcela A.
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Tipo de documento:
Artículo
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Palabras clave:
Helminth
Parasite
Extracellular vesicle
Protein
Marker
Parasite
Extracellular vesicle
Protein
Marker
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Año de publicación:
2023
Contenido:
Resumen:
Helminth parasites cause debilitating—sometimes fatal—diseases in humans and animals.
Despite their impact on global health, mechanisms underlying host–parasite interactions are still poorly understood. One such mechanism involves the exchange of extracellular vesicles (EVs), which are membrane-enclosed subcellular nanoparticles. To date, EV secretion has been studied in helminth parasites, including EV protein content. However, information is highly heterogeneous, since it was generated in multiple species, using varied protocols for EV isolation and data analysis. Here, we compared the protein cargo of helminth EVs to identify common markers for each taxon. For this, we integrated published proteomic data and performed a comparative analysis through an orthology approach. Overall, only three proteins were common in the EVs of the seven analyzed species. Additionally, varied repertoires of proteins with moonlighting activity, vaccine antigens, canonical and non-canonical proteins related to EV biogenesis, taxon-specific proteins of unknown function and RNA-binding proteins were observed in platyhelminth and nematode EVs. Despite the lack of consensus on EV isolation protocols and protein annotation, several proteins were shown to be consistently detected in EV preparations from organisms at different taxa levels, providing a starting point for a selective biochemical characterization.
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EN:
Life. 13(12), 2023
Financiadores:
Agencia Nacional de Investigación e Innovación (ANII)
Citación:
ANCAROLA, ME., MALDONADO, LL., GARCÍA, LCA., y otros. A Comparative Analysis of the Protein Cargo of Extracellular Vesicles from Helminth Parasites. Life [en línea] 2023, 13(12). DOI: 10.3390/life13122286
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Licencia Creative Commons Atribución (CC - By 4.0)
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| Ficheros | Descripción | Tamaño | Formato | ||
|---|---|---|---|---|---|
| A Comparative Analysis of the Protein Cargo of Extracellular Vesicles from Helminth Parasites.pdf | Artículo original | 1.84 MB | Adobe PDF |