Título:
Proliferating toward sex: characterization of cell division of Toxoplasma gondii’s pre-sexual stages
Otros títulos:
Coordinador:
Director:
Compilador:
Autor:
Sena, Florencia
Hakimi, Mohamed-Ali
Francia, Maria E.
Hakimi, Mohamed-Ali
Francia, Maria E.
Tutor:
Tipo de documento:
Artículo
Editor:
Palabras clave:
apicomplexan
Toxoplasma gondii
cell division
merozoite
pre-sexual
endopolygeny
expansion microscopy
Toxoplasma gondii
cell division
merozoite
pre-sexual
endopolygeny
expansion microscopy
Descriptores:
Año de publicación:
2026
Contenido:
Resumen:
Toxoplasmosis is a disease of worldwide distribution, causing high morbidity and mortality in humans, as well as heavily impacting animal health and the economy. Toxoplasma gondii, the causative agent, is an intracellular parasite with a complex life cycle whose completion entails asexual, pre-sexual, and sexual stage conversions. Pre-sexual and sexual differentiation take place only within the intestinal epithelium of felines. Recently, several transcriptional factors and epigenetic compo nents crucial to trigger parasite stage transitions within the cat have been identified, allowing, through precise genetic manipulation, obtaining pre-sexual stages known as merozoites in vitro. Through conditional depletion of two pre-sexual stage-specific gene silencing transcription factors, AP2XII-1 and AP2XII-2, we have characterized the interplay between cell division and the sequence of events leading up to differentiation of tachyzoites into merozoites. We explored genome duplication, assembly of daughter cells, karyokinesis, and cytokinesis, characterizing the differential cell division modes and kinetics undergone by critical structures along the differentiation axis. Building onto the pre-existing body of knowledge, primarily describing the underpinnings of these forms of division by transmission electron microscopy, our work contributes previously unexplored temporal and spatial resolution to the transitions between endodyogeny and endopolygeny, providing a conceptual framework for understanding and exploring T. gondii’s route of sexual differentiation.
Descripción:
metadata.articulos.dc.description.uri:
Editorial:
EN:
mBio. 17(6), 2026
Financiadores:
Fondo para la Convergencia Estructural del Mercosur (FOCEM)
Red Pasteur
Red Pasteur
Citación:
SENA, F., HAKIMI, MA., y FRANCIA, ME. Proliferating toward sex: characterization of cell division of Toxoplasma gondii’s pre-sexual stages. mBio [en línea] 2026,17(6). DOI: 10.1128/mbio.02440-25
Citación:
metadata.articulos.cc.license.name:
ISBN:
e-ISBN:
ISSN:
ISMN:
Otros identificadores:
Cobertura geográfica:
Cobertura temporal:
Departamento académico:
Grupo de investigación:
Licencia:
Licencia Creative Commons Atribución (CC - By 4.0)
Colecciones:
| Ficheros | Descripción | Tamaño | Formato | ||
|---|---|---|---|---|---|
| Proliferating toward sex characterization.pdf | Artículo original | 2.92 MB | Adobe PDF |