Título:
Fast and cost-effective SARS-CoV-2 variant detection using Oxford Nanopore full-length spike gene sequencing
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Autor:
Salazar González, María Cecilia
Ferrés Cáceres, Ignacio
Paz, Mercedes
Costábile Cristech, Alicia
Moratorio, Gonzalo
Moreno Karlen, María del Pilar
Iraola, Gregorio
Ferrés Cáceres, Ignacio
Paz, Mercedes
Costábile Cristech, Alicia
Moratorio, Gonzalo
Moreno Karlen, María del Pilar
Iraola, Gregorio
Tutor:
Tipo de documento:
Artículo
Editor:
Palabras clave:
SARS-CoV-2.
Spike gene
Oxford Nanopore Technologies
Surveillance
Spike gene
Oxford Nanopore Technologies
Surveillance
Descriptores:
Año de publicación:
2023
Contenido:
Resumen:
Most biologically relevant and diagnostic mutations in the severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) genome have been identified in the S gene through global genomic surveillance efforts. However, large-scale whole-genome sequencing (WGS) is still challenging in developing countries due to higher costs, reagent delays and limited infrastructure. Consequently, only a small fraction of SARS-CoV-2 samples are characterized through WGS in these regions. Here, we present a complete workflow consisting of a fast library preparation protocol based on tiled amplification of the S gene, followed by a PCR barcoding step and sequencing using Nanopore platforms. This protocol facilitates fast and cost-effective identification of main variants of concern and mutational surveillance of the S gene. By applying this protocol, report time and overall costs for SARS-CoV-2 variant detection could be reduced, contributing to improved genomic surveillance programmes, particularly in low-income regions.
Descripción:
Editorial:
Microbiology Society
EN:
Microbial Genomics, 2023, 9(5): 001013.
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Citación:
Salazar González, M, Ferrés Cáceres, I, Paz, M [y otros autores]. "Fast and cost-effective SARS-CoV-2 variant detection using Oxford Nanopore full-length spike gene sequencing". Microbial Genomics. [en línea] 2023, 9(5): 001013. 10 h. DOI: 10.1099/mgen.0.001013.
Citación:
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Documento del Gobiberno:
URI:
https://hdl.handle.net/20.500.12008/42822
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ISSN:
2057-5858
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Licencia:
Licencia Creative Commons Atribución (CC - By 4.0)
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| Ficheros | Descripción | Tamaño | Formato | ||
|---|---|---|---|---|---|
| 10.1099.mgen.0.001013.pdf | — | 2.77 MB | Adobe PDF |