Por favor, use este identificador para citar o enlazar este ítem: https://hdl.handle.net/20.500.12008/42441

Título:

A multiplex-NGS approach to identifying respiratory RNA viruses during the COVID-19 pandemic

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Autor:

Ramos, Natalia
Panzera Crespo, Yanina
Frabasile Giurato, Sandra Alicia
Tomás Custodio, Gonzalo Martín
Calleros Basilio, Lucía
Marandino, Ana
Techera, Claudia
Grecco Patiño, Sofía
Fuques Villalba, Eddie
Arbiza, Juan
Pérez Crossa, Ruben Gustavo
Delfraro Vázquez, Adriana Beatriz
Goñi Mazzitelli, Natalia
Coppola, Leticia
Ramas, Vivivana
Morel, Noelia
Chiparelli, Héctor

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Tipo de documento:

Artículo

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Palabras clave:

COVID-19 pandemic
SARS-CoV-2
Human respiratory RNA viruses
Multiplex PCR-NGS
Viral coinfections

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Año de publicación:

2023

Contenido:

Resumen:

A methodological approach based on reverse transcription (RT)-multiplex PCR followed by next-generation sequencing (NGS) was implemented to identify multiple respiratory RNA viruses simultaneously. A convenience sampling from respiratory surveillance and SARS-CoV-2 diagnosis in 2020 and 2021 in Montevideo, Uruguay, was analyzed. The results revealed the cocirculation of SARS-CoV-2 with human rhinovirus (hRV) A, B and C, human respiratory syncytial virus (hRSV) B, influenza A virus, and metapneumovirus B1. SARS-CoV-2 coinfections with hRV or hRSV B and influenza A virus coinfections with hRV C were identified in adults and/or children. This methodology combines the benefits of multiplex genomic amplification with the sensitivity and information provided by NGS. An advantage is that additional viral targets can be incorporated, making it a helpful tool to investigate the cocirculation and coinfections of respiratory viruses in pandemic and post-pandemic contexts.

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EN:

Archives of virology, 2023, 168(3): 87.

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Citación:

Ramos, N, Panzera Crespo, Y, Frabasile Giurato, S [y otros autores]. "A multiplex-NGS approach to identifying respiratory RNA viruses during the COVID-19 pandemic". Archives of virology. [en línea] 2023 168(3): 87. 7 h. DOI: 10.1007/s00705-023-05717-6

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ISSN:

1432-8798

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Licencia:

Licencia Creative Commons Atribución (CC - By 4.0)

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Publicaciones académicas y científicas
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