Título:
Requirement of Pax6 for the integration of guidance cues in cell migration
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Autor:
Arocena, Miguel
Rajnicek, A. M.
Collinson, J. M.
Rajnicek, A. M.
Collinson, J. M.
Tutor:
Tipo de documento:
Artículo
Editor:
Palabras clave:
Contact guidance
Electrotaxis
Neural progenitor
Pax6
Electrotaxis
Neural progenitor
Pax6
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Año de publicación:
2017
Contenido:
Resumen:
The intricate patterns of cell migration that are found throughout development are generated through a vast array of guidance cues. Responding integratively to distinct, often conflicting, migratory signals is probably crucial for cells to reach their correct destination. Pax6 is a master transcription factor with key roles in neural development that include the control of cell migration. In this study, we have investigated the ability of cells derived from cortical neurospheres from wildtype (WT) and Pax6-/- mouse embryos to integrate diverging guidance cues. We used two different cues, either separately or in combination: substratum nanogrooves to induce contact guidance, and electric fields (EFs) to induce electrotaxis. In the absence of an EF, both WT and Pax6-/- cells aligned and migrated parallel to grooves, and on a flat substrate both showed marked electrotaxis towards the cathode. When an EF was applied in a perpendicular orientation to grooves, WT cells responded significantly to both cues, migrating in highly oblique trajectories in the general direction of the cathode. However, Pax6-/- cells had an impaired response to both cues simultaneously. Our results demonstrate that these neurosphere derived cells have the capacity to integrate diverging guidance cues, which requires Pax6 function.
Descripción:
Editorial:
Royal Society Publishing
EN:
Royal Society Open Science, 2017, 4 (10), art. no. 170625
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Citación:
Arocena, M., Rajnicek, A.,Collinson, J.M. Requirement of Pax6 for the integration of guidance cues in cell migration. Royal Society Open Science, 2017, 4 (10), art. no. 170625. doi: 10.1098/rsos.170625
Citación:
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URI:
https://hdl.handle.net/20.500.12008/22031
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ISSN:
2054-5703
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Licencia Creative Commons Atribución (CC –BY 4.0)
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| Ficheros | Descripción | Tamaño | Formato | ||
|---|---|---|---|---|---|
| 101098rsos170625.pdf | — | 1.23 MB | Adobe PDF |