Título:
Fosfomycin tromethamine activity on biofilm and intracellular bacterial communities produced by uropathogenic Escherichia coli isolated from patients with urinary tract infection
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Autor:
González, María José
Da Cunda, Paula
Notejane, Martín
Zunino, Pablo
Scavone, Paola
Robino, Luciana
Da Cunda, Paula
Notejane, Martín
Zunino, Pablo
Scavone, Paola
Robino, Luciana
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Tipo de documento:
Artículo
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Palabras clave:
Fosfomycin tromethamine
Intracellular bacterial communities
Biofilms
Uropathogenic E. coli
Urinary tract infections
Antibiotic resistance
Intracellular bacterial communities
Biofilms
Uropathogenic E. coli
Urinary tract infections
Antibiotic resistance
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Año de publicación:
2019
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Resumen:
Fosfomycin tromethamine (FT), an old antibiotic revived as a new strategy to overcome antibiotic resistance, is an excellent option for the treatment of lower urinary tract infection (UTI). During UTI, Escherichia coli produces biofilms and could invade the bladder epithelial cells, developing intracellular bacterial communities (IBC). The present work aimed to evaluate the activity of FT on biofilms and IBC from clinical isolates of E. coli. A total of 38 E. coli clinical UTI isolates previously characterized as biofilm and IBC producers were studied. FT susceptibility was evaluated and its activity on 48 h biofilm was determined by microtiter plate-based biofilm assay comparing three different antibiotic concentrations. Two UPEC strains were selected to evaluate FT activity on IBC in vitro using T24 bladder cells. The survival percentage of intracellular bacteria after 24 h exposure to FT was calculated and compared to the percentage of intracellular bacteria without antibiotic. All the strains were susceptible to FT. FT produced a significant reduction of biofilms at the three concentrations tested, compared to the control. However, no statistically effect on IBC was observed after 24 h of fosfomycin exposure in cell culture. FT is a good option for bacterial biofilm reduction within UTI. However, it does not affect IBC.
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EN:
Pathog. Dis. 77, 2019
Financiadores:
Comisión Sectorial de Investigación Científica (CSIC)
Citación:
GONZÁLEZ, MJ., DA CUNDA, P., NOTEJANE, M., y otros. Fosfomycin tromethamine activity on biofilm and intracellular bacterial communities produced by uropathogenic Escherichia coli isolated from patients with urinary tract infection. Pathog. Dis [en línea] 2019, 77. DOI: 10.1093/femspd/ftz022
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https://hdl.handle.net/20.500.12008/50569
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Licencia Creative Commons Atribución (CC - By 4.0)
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| Ficheros | Descripción | Tamaño | Formato | ||
|---|---|---|---|---|---|
| Fosfomycin tromethamine activity on biofilm and intracellular bacterial communities produced by uropathogenic Escherichia coli isolated from patients with urinary tract infection.pdf | Artículo original | 3.66 MB | Adobe PDF |