Por favor, use este identificador para citar o enlazar este ítem: https://hdl.handle.net/20.500.12008/55155

Título:

HPLC-MS/MS Oxylipin Analysis of Plasma from Amyotrophic Lateral Sclerosis Patients

Otros títulos:

Coordinador:

Director:

Compilador:

Autor:

Mastrogiovanni, Mauricio
Trostchansky, Andrés
Naya, Hugo
Domínguez, Raúl
Marco, Carla
Povedano, Mónica
López-Vales, Rubén
Rubbo, Homero

Tutor:

Tipo de documento:

Artículo

Editor:

Palabras clave:

Amyotrophic lateral sclerosis
Lipidomics
Mass spectrometry
Oxylipin
Specialized pro-resolving mediators

Descriptores:

CROMATOGRAFÍA LÍQUIDA CON ESPECTROMETRÍA DE MASAS
ESCLEROSIS AMIOTRÓFICA LATERAL
OXILIPINAS
PLASMA
LIPIDÓMICA
ESPECTOMETRÍA DE MASAS
MEDIADORES PRO RESOLUCIÓN ESPECIALIZADOS

Año de publicación:

2022

Contenido:

Resumen:

Oxylipins play a critical role in regulating the onset and resolution phase of inflammation. Despite inflammation is a pathological hallmark in amyotrophic lateral sclerosis (ALS), the plasma oxylipin profile of ALS patients has not been assessed yet. Herein, we develop an oxylipin profile-targeted analysis of plasma from 74 ALS patients and controls. We found a significant decrease in linoleic acid-derived oxylipins in ALS patients, including 9-hydroxy-octadecadienoic acid (9-HODE) and 13-HODE. These derivatives have been reported as important regulators of inflammation on different cell systems. In addition, some 5-lipoxygenase metabolites, such as 5-hydroxy- eicosatetraenoic acid also showed a significant decrease in ALS plasma samples. Isoprostanes of the F2α family were detected only in ALS patients but not in control samples, while the hydroxylated metabolite 11-HETE significantly decreased. Despite our effort to analyze specialized pro-resolving mediators, they were not detected in plasma samples. However, we found the levels of 14-hydroxy-docosahexaenoic acid, a marker pathway of the Maresin biosynthesis, were also reduced in ALS patients, suggesting a defective activation in the resolution programs of inflammation in ALS. We further analyze oxylipin concentration levels in plasma from ALS patients to detect correlations between these metabolites and some clinical parameters. Interestingly, we found that plasmatic levels of 13-HODE and 9-HODE positively correlate with disease duration, expressed as days since onset. In summary, we developed a method to analyze "(oxy)lipidomics" in ALS human plasma and found new profiles of metabolites and novel lipid derivatives with unknown biological activities as potential footprints of disease onset.

Descripción:

metadata.articulos.dc.description.uri:

Editorial:

EN:

Biomedicines. 2022;10(3)

Financiadores:

Citación:

Mastrogiovanni M, Trostchansky A, Naya H y otros. HPLC-MS/MS Oxylipin Analysis of Plasma from Amyotrophic Lateral Sclerosis Patients. Biomedicines [en línea]. 2022;10(3). 13 p.

Citación:

metadata.articulos.cc.license.name:

ISBN:

e-ISBN:

ISSN:

ISMN:

Otros identificadores:

Cobertura geográfica:

Cobertura temporal:

Departamento académico:

Grupo de investigación:

Licencia:

Licencia Creative Commons Atribución (CC - By 4.0)

Colecciones:

Publicaciones Académicas y Científicas
FicherosDescripciónTamañoFormato
HPLC MS MS Oxylipin Analysis of Plasma from Amyotrophic.pdf HPLC MS MS Oxylipin Analysis of Plasma from Amyotrophic 5.83 MB Adobe PDF
Mostrar el registro Dublin Core completo del ítem