| Metadato | Etiqueta | Valor | Idioma |
|---|---|---|---|
| dc.contributor.author | Autor | Arbiza, Juan | - |
| dc.contributor.author | Autor | Castro, E. R. | - |
| dc.contributor.filiacion | Filiación | Arbiza Juan, Universidad de la República (Uruguay). Facultad de Ciencias. Instituto de Biología. | - |
| dc.contributor.filiacion | Filiación | Castro E. R. | - |
| dc.date.accessioned | Fecha ingreso | 2022-10-13T12:29:36Z | - |
| dc.date.available | Fecha disponible | 2022-10-13T12:29:36Z | - |
| dc.date.issued | Año de publicación | 2017 | - |
| dc.description.abstract | Resumen | Infection with equid alphaherpesvirus 1 (EHV-1) causes respiratory disease, abortion and neurological disorders in horses. Molecular epidemiology studies have demonstrated that a single nucleotide polymorphism (A2254/G2254) in the genome region of open reading frame 30 which results in an amino acid variation (N752/D752) of the EHV-1 DNA polymerase, is significantly associated with the neuropathogenic potential of naturally occurring strains. In recent years, an increase in the number of cases of equine neurological disease caused by neuropathogenic variants of EHV-1 has been observed in numerous countries. The purpose of this study was to detect the presence of the viral genome of EHV-1 and equid herpesvirus 4 (EHV-4) in the bronchopulmonary lymph nodes of 47 horses from various locations in Uruguay, obtained from a slaughterhouse, and to determine whether the EHV-1 genomes possessed the mutation associated with neuropathogenesis (G2254/D752). The genes encoding glycoprotein H (gH) of EHV-1 and glycoprotein B (gB) of EHV-4 were amplified by a semi-nested polymerase chain reaction. Of the samples analysed, 28% and 6% of lymph nodes contained the genes for gH and gB, respectively. The viral DNA olymerase gene was amplified and sequenced. Twelve of the 13 genomes sequenced presented the nucleotide G2254, while the remaining 1 showed both nucleotides, A2254 and G2254. The results confirm the presence of EHV-1 in Uruguay. Furthermore, there is evidence for the first time of the detection of EHV- 4, and high-frequency detection of the neuropathogenic variant (G2254/D752) of EHV-1 in Uruguay. These findings provide new insights into the epidemiological situation of EHV-1 and EHV-4 in that country. | es |
| dc.format.extent | Extensión | 8 h. | es |
| dc.format.mimetype | Formato | application/pdf | es |
| dc.identifier.citation | Citación | Arbiza, J. y Castro, E. "Detection and genotyping of equid herpesvirus 1 in Uruguay". OIE Revue Scientifique et Technique. [en línea] 2017, 36 (3): 799-806. 8 h. | es |
| dc.identifier.doi | DOI | 10.20506/rst36.3.2715 | - |
| dc.identifier.issn | ISSN | 0253-1933 | - |
| dc.identifier.uri | URI | https://hdl.handle.net/20.500.12008/34150 | - |
| dc.language.iso | Idioma | en_US | es |
| dc.publisher | Editorial | Office International des Epizootes | es |
| dc.relation.ispartof | EN | OIE Revue Scientifique et Technique, 2017, 36 (3): 799-806. | es |
| dc.rights | Derechos | Las obras depositadas en el Repositorio se rigen por la Ordenanza de los Derechos de la Propiedad Intelectual de la Universidad de la República.(Res. Nº 91 de C.D.C. de 8/III/1994 – D.O. 7/IV/1994) y por la Ordenanza del Repositorio Abierto de la Universidad de la República (Res. Nº 16 de C.D.C. de 07/10/2014) | es |
| dc.rights.licence | Licencia | Licencia Creative Commons Atribución - No Comercial - Sin Derivadas (CC - By-NC-ND 4.0) | es |
| dc.subject | Palabras clave | Equid herpesvirus | es |
| dc.subject | Palabras clave | Genotyping | es |
| dc.subject | Palabras clave | Lymph node | es |
| dc.subject | Palabras clave | Molecular detection | es |
| dc.subject | Palabras clave | Neuropathogenic | es |
| dc.subject | Palabras clave | Uruguay | es |
| dc.title | Título | Detection and genotyping of equid herpesvirus 1 in Uruguay | es |
| dc.type | Tipo de documento | Artículo | es |
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| Ficheros | Descripción | Tamaño | Formato | ||
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| 10.20506rst36.3.2715.pdf | — | 244.96 KB | Adobe PDF |