Título:
Pregnancy and Bovine Somatotropin in Nonlactating Dairy Cows: II. Endometrial Gene Expression Related to Maintenance of Pregnancy
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Autor:
Guzeloglu, A.
Bilby, T.R.
Meikle, Ana
Kamimura, S.
Kowalski, A.
Michel, F.
MacLaren, L.A.
Thatcher, W.W.
Bilby, T.R.
Meikle, Ana
Kamimura, S.
Kowalski, A.
Michel, F.
MacLaren, L.A.
Thatcher, W.W.
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Artículo
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BOVINOS
VACAS LECHERAS
GESTACION
ENDOMETRIO
SOMATOTROPINA BOVINA
VACAS LECHERAS
GESTACION
ENDOMETRIO
SOMATOTROPINA BOVINA
Año de publicación:
2004
Contenido:
Resumen:
The objective was to evaluate the effects of pregnancy and bovine somatotropin (bST) on endometrial gene and protein expression related to maintenance of pregnancy in nonlactating dairy cows at d 17. In endometrial tissues, treatment with bST increased the steady state concentration of oxytocin receptor (OTR) mRNA; bSTtreated cyclic (bST-C) cows had greater OTR mRNA than bST-treated pregnant (bST-P) cows. Estradiol receptor α (ERα) mRNA was reduced in bST-P cows compared with control P and C (no bST) cows. Western blotting revealed that pregnancy decreased the abundance of ERα protein, and bST stimulated an increase in ERα protein in C and P cows. Treatment with bST increased steady state concentrations of progesterone receptor (PR) mRNA. No differences were detected in steady state mRNA concentrations of prostaglandin H synthase-2 (PGHS-2), prostaglandin E synthase, and prostaglandin F synthase due to pregnancy or bST treatment. However, PGHS-2 protein was increased in response to pregnancy and bST treatment. Immunostaining indicated that P decreased ERα protein in luminal epithelium and increased PR protein in epithelial cells of the uterine glands. The PR protein response in the glands was less in bST-P cows than in P cows. In the stromal layer of the endometrium, bST decreased PR protein abundance in C and P cows. The PGHS-2 protein was localized exclusively in the luminal epithelium cells of endometrium and was increased in P cows. In conclusion, distinctly different mRNA and protein responses were detected between C and P cows related to prostaglandin biosynthesis, and bST-induced changes may potentially impact mechanisms associated with maintenance of pregnancy in nonlactating cows.
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Journal of Dairy Science, 2004, 87(10), 3268–3279
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Citación:
Guzeloglu, A, Bilby, T, Meikle, A, Kamimura, S, Kowalski, A, Michel, F, MacLaren, L y Thatcher, W. Pregnancy and Bovine Somatotropin in Nonlactating Dairy Cows: II. Endometrial Gene Expression Related to Maintenance of Pregnancy. Journal of Dairy Science. [en línea] 2004, 87(10), 3268–3279
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Licencia Creative Commons Atribución - No Comercial - Sin Derivadas (CC - By-NC-ND 4.0)
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| M30.pdf | — | 829.77 KB | Adobe PDF |