Título:
Macrophage IL-4 polarization is restricted by soluble CD40 agonists and antigen-induced CD154 but not by constitutive CD154 expressed by CD4+ T cells
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Autor:
Suárez-Martins, Mariana
González-Alayón, Ignacio
Casaravilla, Cecilia
Boon, Louis
Papotto, Pedro H.
Finlay, Conor M.
Jenkins, Stephen J.
Allen, Judith E.
Díaz, Álvaro
González-Alayón, Ignacio
Casaravilla, Cecilia
Boon, Louis
Papotto, Pedro H.
Finlay, Conor M.
Jenkins, Stephen J.
Allen, Judith E.
Díaz, Álvaro
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Tipo de documento:
Preprint
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Palabras clave:
CD40
CD154
Macrófago
Cavidad peritoneal
CD154
Macrófago
Cavidad peritoneal
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Año de publicación:
2025
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Resumen:
Stimulation of macrophages via CD40 promotes their classical activation. Therefore, CD154 (CD40 ligand) can be expected to oppose macrophage polarization and proliferation induced by IL-4. However, there is limited experimental data to support this, which is additionally complicated by the possibility of differential effects of CD40 agonists in different formats/contexts. Whereas canonically CD4+ T cells upregulate CD154 strongly following exposure to cognate antigen, naïve CD4+ cells constitutively express significant levels of CD154, which could be a tonic signal. Soluble CD154 and agonistic CD40 antibodies also trigger CD40 signaling. We explored these questions in a reductionist model of IL-4 delivery to mouse peritoneal cavity cells in vitro and in vivo. Soluble CD40 agonists inhibited M(IL-4) polarization, with a stronger effect on RELMa than on Ym1 (Chil3), as well as inhibiting IL-4 induced proliferation. CD154 provided by CD4+ cells in the context of an antigen-specific interaction blunted macrophage RELM-a expression but did not affect Ym1. Macrophages negatively regulated, via CD40, constitutive cell-surface CD154 on naïve CD4+ cells, both in vitro and in vivo. The large peritoneal macrophages of CD40 KO mice showed a moderately enhanced RELMa response to IL-4, but this was not a cell-autonomous effect. No differences between WT and CD40 KO mice were detected in IL-4-induced macrophage proliferation. We conclude that strong CD40 stimulation, included stimulation by CD154 expressed by antigen-specific CD4+ cells, blunts selected macrophage responses to IL-4, and that constitutive CD4+-cell CD154, in spite of interacting with CD40 on macrophages, does not directly influence macrophage responses to IL-4.
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Oxford University Press
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Journal of Leukocyte Biology, v. 117, n°7, 2025. -- eqiaf069
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Citación:
Suárez-Martins, M., González-Alayón, I., Casaravilla, C. y otros. "Macrophage IL-4 polarization is restricted by soluble CD40 agonists and antigen-induced CD154 but not by constitutive CD154 expressed by CD4+ T cells" [Preprint]. Journal of Leukocyte Biology [en línea] v. 117, n°7, 2025. -- eqiaf069. 75 p.
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Licencia Creative Commons Atribución - No Comercial - Sin Derivadas (CC - By-NC-ND 4.0)
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| Ficheros | Descripción | Tamaño | Formato | ||
|---|---|---|---|---|---|
| Macrophage.pdf | — | 6.72 MB | Adobe PDF |