Título:
Jejunum‑derived NF‑κB reporter organoids as 3D models for the study of TNF‑alpha‑induced inflammation
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Daghero Villanueva, Hellen
Doffe, Flora
Varela Cruces, María Belén
Yozzi, Victoria
Verdes García, José Manuel
Crispo, Martina
Bollati-Fogolín, Mariela
Pagotto, Romina
Doffe, Flora
Varela Cruces, María Belén
Yozzi, Victoria
Verdes García, José Manuel
Crispo, Martina
Bollati-Fogolín, Mariela
Pagotto, Romina
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RATONES
VIROSIS
HEPATITIS
TERAPIA
VIROSIS
HEPATITIS
TERAPIA
Año de publicación:
2022
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Resumen:
Inflammation is an important process for epithelial barrier protection but when uncontrolled, it can also lead to tissue damage. The nuclear factor-kappa light chain enhancer of activated B cells (NF-κB) signaling pathway is particularly relevant in the intestine, as it seems to play a dual role. Whereas NF-κB protects intestinal epithelium against various noxious stimuli, the same pathway mediates intestinal inflammatory diseases by inducing pro-inflammatory gene expression. The availability of appropriate in vitro models of the intestinal epithelium is crucial for further understanding the contribution of NF-κB in physiological and pathological processes and advancing in the development of drugs and therapies against gut diseases. Here we established, characterized, and validated three-dimensional cultures of intestinal organoids obtained from biopsies of NF-κB-RE-Luc mice. The NF-κB-RE-Luc intestinal organoids derived from different intestine regions recreated the cellular composition of the tissue and showed a reporter responsiveness similar to the in vivo murine model. When stimulated with TNF-α, jejunum-derived NF-κB-RE-Luc-reporter organoids, provided a useful model to evaluate the anti-inflammatory effects of natural and synthetic compounds. These reporter organoids are valuable tools to explore the epithelial TNF-α-induced NF-κB contribution in the small intestine, being a reliable alternative method while helping to reduce the use of laboratory animals for experimentation.
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Scientific Reports, 2022, 12(14425), 1-14
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Daghero Villanueva, H, Doffe, F, Varela Cruces, M, Yozzi, V, Verdes García, J, Crispo, M, Bollati-Fogolín, M y Pagotto, R. Jejunum‑derived NF‑κB reporter organoids as 3D models for the study of TNF‑alpha‑induced inflammation. Scientific Reports. [en línea] 2022, 12(14425), 1-14
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Licencia Creative Commons Atribución (CC - By 4.0)
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| Ficheros | Descripción | Tamaño | Formato | ||
|---|---|---|---|---|---|
| V5.pdf | — | 2.2 MB | Adobe PDF |