Título:
Biomolecular condensates modulate membrane lipid packing and hydration
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Autor:
Mangiarotti, Agustín
Siri, Macarena
Tam, Nicky W.
Zhao, Ziliang
Malacrida, Leonel
Dimova, Rumiana
Siri, Macarena
Tam, Nicky W.
Zhao, Ziliang
Malacrida, Leonel
Dimova, Rumiana
Tutor:
Tipo de documento:
Artículo
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Descriptores:
CONDENSADOS BIOMOLECULARES
LÍPIDOS DE LA MEMBRANA
BIOLOGÍA CELULAR
MICROSCOPÍA
IMÁGENES HIPERESPECTRALES
AMINOÁCIDOS
ESTRUCTURA SECUNDARIA DE PROTEÍNA
LÍPIDOS DE LA MEMBRANA
BIOLOGÍA CELULAR
MICROSCOPÍA
IMÁGENES HIPERESPECTRALES
AMINOÁCIDOS
ESTRUCTURA SECUNDARIA DE PROTEÍNA
Año de publicación:
2023
Contenido:
Resumen:
Membrane wetting by biomolecular condensates recently emerged as a key phenomenon in cell biology, playing an important role in a diverse range of processes across different organisms. However, an understanding of the molecular mechanisms behind condensate formation and interaction with lipid membranes is still missing. To study this, we exploited the properties of the dyes ACDAN and LAURDAN as nano-environmental sensors in combination with phasor analysis of hyperspectral and lifetime imaging microscopy. Using glycinin as a model condensate-forming protein and giant vesicles as model membranes, we obtained vital information on the process of condensate formation and membrane wetting. Our results reveal that glycinin condensates display differences in water dynamics when changing the salinity of themedium as a consequence of rearrangements in the secondary structure of the protein. Remarkably, analysis of membrane-condensates interaction with protein as well as polymer condensates indicated a correlation between increasedwetting affinity and enhanced lipid packing. This is demonstrated by a decrease in the dipolar relaxation of water across all membrane-condensate systems, suggesting a general mechanism to tune membrane packing by condensate wetting.
Descripción:
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Editorial:
Nature Research
EN:
Nature Communications. 2023;14(1)
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Citación:
Mangiarotti A, Siri M, Tam N y otros. Biomolecular condensates modulate membrane lipid packing and hydration. Nature Communications [en línea]. 2023;14(1). 19 p.
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ISSN:
2041-1723
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Licencia Creative Commons Atribución (CC - By 4.0)
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| Ficheros | Descripción | Tamaño | Formato | ||
|---|---|---|---|---|---|
| Biomolecular condensates modulate.pdf | Biomolecular condensates modulate | 34.26 MB | Adobe PDF |