Título:
Signal-regulatory protein alpha is an anti-viral entry factor targeting viruses using endocytic pathways
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Autor:
Sarute, Nicolás
Cheng, Han
Yan, Zhonghao
Salas-Briceno, Karen
Richne, Justin
Rong, Lijun
Ross, S. R.
Cheng, Han
Yan, Zhonghao
Salas-Briceno, Karen
Richne, Justin
Rong, Lijun
Ross, S. R.
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Tipo de documento:
Artículo
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Palabras clave:
Vesicular stomatitis virus
Ebola virus
Small interfering RNA
Phagocytosis
SARS CoV 2
293T cells
Transfection
Machupo Virus
Ebola virus
Small interfering RNA
Phagocytosis
SARS CoV 2
293T cells
Transfection
Machupo Virus
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Año de publicación:
2021
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Resumen:
Signal-regulatory protein alpha (SIRPA) is a well-known inhibitor of phagocytosis when it complexes with CD47 expressed on target cells. Here we show that SIRPA decreased in vitro infection by a number of pathogenic viruses, including New World and Old World arena-viruses, Zika virus, vesicular stomatitis virus and pseudoviruses bearing the Machupo virus, Ebola virus and SARS-CoV-2 glycoproteins, but not HSV-1, MLV or mNoV. Moreover, mice with targeted mutation of the Sirpa gene that renders it non-functional were more suscepti
ble to infection with the New World arenaviruses Junı´n virus vaccine strain Candid 1 and Tacaribe virus, but not MLV or mNoV. All SIRPA-inhibited viruses have in common the requirement for trafficking to a low pH endosomal compartment. This was clearly demonstrated with SARS-CoV-2 pseudovirus, which was only inhibited by SIRPA in cells in which it required trafficking to the endosome. Similar to its role in phagocytosis inhibition, SIRPA
decreased virus internalization but not binding to cell surface receptors. We also found that increasing SIRPA levels via treatment with IL-4 led to even greater anti-viral activity. These data suggest that enhancing SIRPA’s activity could be a target for anti-viral therapies.
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PloS ONE
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PLoS Pathog,2021, 17(6): e1009662.
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Citación:
Sarute, N, Cheng, H, Yan, Z, [y otros autores] "Signal-regulatory protein alpha is an anti-viral entry factor targeting viruses using endocytic pathways". PLoS Pathog. [en línea] 2021, 17(6): e1009662. 23 h. DOI: 10.1371/journal.ppat.1009662
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https://hdl.handle.net/20.500.12008/38247
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1553-7374
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Licencia Creative Commons Atribución (CC - By 4.0)
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| Ficheros | Descripción | Tamaño | Formato | ||
|---|---|---|---|---|---|
| 101371journalppat1009662.pdf | — | 532.35 KB | Adobe PDF |