Título:
Hsa-miR-183-5p modulates cell adhesion by repression of ITGB1 expression in prostate cancer
Otros títulos:
Coordinador:
Director:
Compilador:
Autor:
Oliveira-Rizzo, Carolina
Ottati Braselli, María Carolina
Fort Canobra, Rafael S
Chávez, Santiago
Trinidad Barnech, Juan Manuel
Di Paolo, Andrés
Garat, Beatriz
Sotelo-Silveira, José Roberto
Duhagon, María Ana
Ottati Braselli, María Carolina
Fort Canobra, Rafael S
Chávez, Santiago
Trinidad Barnech, Juan Manuel
Di Paolo, Andrés
Garat, Beatriz
Sotelo-Silveira, José Roberto
Duhagon, María Ana
Tutor:
Tipo de documento:
Artículo
Editor:
Palabras clave:
Cancer
MicroRNA
Focal adhesion
miR-183
Prostate
ITGB
TCGA
AGO-PAR-CLIP
MicroRNA
Focal adhesion
miR-183
Prostate
ITGB
TCGA
AGO-PAR-CLIP
Descriptores:
Año de publicación:
2022
Contenido:
Resumen:
Prostate cancer is a major health problem worldwide. MiR-183 is an oncomiR and a candidate biomarker in prostate cancer, affecting various pathways responsible for disease initiation and progression. We sought to discover the most relevant processes controlled by miR-183 through an unbiased transcriptomic approach using prostate cell lines and patient tissues to identify miR-183 responsive genes and pathways. Gain of unction experiments, reporter gene assays, and transcript and protein measurements were conducted to validate predicted functional effects and protein mediators. A total of 135 candidate miR-183 target genes overrepresenting cell adhesion terms were inferred from the integrated transcriptomic analysis. Cell attachment, spreading assays and focal adhesion quantification of miR-183-overexpressing cells confirmed the predicted reduction in cell adhesion. ITGB1 was validated as a major target of repression by miR-183 as well as a mediator of cell adhesion in response to miR-183. The reporter gene assay and PAR-CLIP read mapping
suggest that ITGB1 may be a direct target of miR-183. The negative correlation between miR-183 and ITGB1 expression in prostate cancer cohorts supports their interaction in the clinical set. Overall, cell adhesion was uncovered as a major pathway controlled by miR-183 in prostate cancer, and ITGB1 was identified as a relevant mediator of this effect.
Descripción:
metadata.articulos.dc.description.uri:
Editorial:
MDPI
EN:
Non-Coding RNA, 2022, 8(1): 11.
Financiadores:
CSIC: I+D 2016_487
CSIC: I+D 2020_566
CSIC: I+D 2020_566
Citación:
Oliveira-Rizzo, C, Ottati Braselli, M, Fort Canobra, R. y otros. "Hsa-miR-183-5p modulates cell adhesion by repression of ITGB1 expression in prostate cancer". Non-Coding RNA. [en línea] 2022, 8(1): 11.21 h. DOI: 10.3390/ncrna8010011
Citación:
metadata.articulos.cc.license.name:
ISBN:
e-ISBN:
ISSN:
2311-553X
ISMN:
Otros identificadores:
Cobertura geográfica:
Cobertura temporal:
Departamento académico:
Grupo de investigación:
Licencia:
Licencia Creative Commons Atribución (CC - By 4.0)
| Ficheros | Descripción | Tamaño | Formato | ||
|---|---|---|---|---|---|
| 103390ncrna8010011.pdf | — | 2.06 MB | Adobe PDF |