Título:
Hypoxia, acidification and oxidative stress in cells cultured at large distances from an oxygen source
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Autor:
D’Aiuto, Natali
Hochmann Valls, Jimena
Millán, Magdalena
Di Paolo, Andrés
Bologna-Molina, Ronell
Sotelo-Silveira, José Roberto
Arocena, Miguel
Hochmann Valls, Jimena
Millán, Magdalena
Di Paolo, Andrés
Bologna-Molina, Ronell
Sotelo-Silveira, José Roberto
Arocena, Miguel
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HIPOXIA
ESTRES OXIDATIVO
HIPOXIA DE LA CELULA
OXIGENO
INGENIERIA DE TEJIDOS
ESTRES OXIDATIVO
HIPOXIA DE LA CELULA
OXIGENO
INGENIERIA DE TEJIDOS
Año de publicación:
2022
Contenido:
Resumen:
Hypoxia is a condition frequently encountered by cells in tissues, whether as a normal feature of their microenvironment or subsequent to deregulated growth. Hypoxia can lead to acidifcation and increased oxidative stress, with profound consequences for cell physiology and tumorigenesis. Therefore, the interplay between hypoxia and oxidative stress is an important aspect for understanding the efects of hypoxic microenvironments on cells. We have used a previously developed variant of the method of coverslip-induced hypoxia to study the process of acidifcation in a hypoxic microenvironment and to simultaneously visualize intracellular levels of hypoxia and oxidative stress. We observed high accumulation of CO2 in hypoxic conditions, which we show is
the main contributor to acidifcation in our model. Also, increased levels of oxidative stress were observed in moderately hypoxic cells close to the oxygen source, where the mitochondrial membrane potential was preserved. Conversely, cells at large distances from the oxygen source showed higher levels of hypoxia, milder oxidative stress and reduced mitochondrial membrane potential. Our results contribute to characterize the interplay between reduced oxygen levels, acidifcation and oxidative stress in a simple in vitro setting, which can be used to model cell responses to an altered environment, such as the early tumor microenvironment.
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Scientifc Reports 2022, 12:21699
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Citación:
D’Aiuto, N, Hochmann, J, Millán, M y otros. Hypoxia, acidification and oxidative stress in cells cultured at large distances from an oxygen source. Scientifc Reports [en línea] 2022 12:21699 ..doi./10.1038/s41598-022-26205-y
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Licencia Creative Commons Atribución - No Comercial (CC - By-NC 4.0)
Colecciones:
| Ficheros | Descripción | Tamaño | Formato | ||
|---|---|---|---|---|---|
| Hypoxia.pdf | Con modificación del resumen | 661.73 KB | Adobe PDF | ||
| HypoxiaNatali D’Aiuto.pdf | — | 1.52 MB | Adobe PDF |