Título:
Nanobody-based blocking of binding ELISA for the detection of anti-NS1 Zika-Virus-Specific antibodies in convalescent patients
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Autor:
Delfin-Riela, Triana
Rossotti, Martín A.
Mattiuzzo, Giada
Echaides, César
González-Sapienza, Gualberto
Rossotti, Martín A.
Mattiuzzo, Giada
Echaides, César
González-Sapienza, Gualberto
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Tipo de documento:
Artículo
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Descriptores:
SEROLOGIA
FLAVIVIRIDAE
INMUNOENSAYO
ANTICUERPOS
ZIKA
FLAVIVIRIDAE
INMUNOENSAYO
ANTICUERPOS
ZIKA
Año de publicación:
2023
Contenido:
Resumen:
Zika virus has spread around the world with rapid pace in the last five years. Although symptoms are typically mild and unspecific, Zika’s major impact occurs during pregnancy, generating a congenital syndrome. Serology plays a key role in its diagnosis. However, its use is limited due to the uncertainty caused by the cross-reaction of antibodies elicited in response to other flavivirus infections when tested in direct immunoassays. Using a panel of previously generated anti-Zika non-structural protein 1 (NS1) nanobodies, a set was selected that only recognizes epitopes present in Zika and is immunogenic to humans. A proper arrangement of these nanobodies was made and conditions were optimized in order to develop a novel serology assay. This new ELISA relies on the inhibition of the binding of a set of selected nanobodies to Zika-immobilized NS1 when previously incubated with Zika convalescent sera. Using the developed blocking of binding assay, it was possible to discriminate between Zika-specific and cross-reactive antibodies in serum samples from infections with Zika and other flaviviruses.
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MDPI
EN:
Tropical Medicine and Infectious Disease, v.8, n° 1, 2023. -- e55
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Citación:
Delfin-Riela, T, Rossotti, M, Mattiuzzo, G, y otros. "Nanobody-based blocking of binding ELISA for the detection of anti-NS1 Zika-Virus-Specific antibodies in convalescent patients". Tropical Medicine and Infectious Disease. [en línea] 2023 vol.8, n° 1, e55. DOI: https://doi.org/10.3390/tropicalmed8010055
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Licencia Creative Commons Atribución (CC - By 4.0)
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| Ficheros | Descripción | Tamaño | Formato | ||
|---|---|---|---|---|---|
| AA Delfin-Riela, T. et al Tropical Medicine and Infectious Disease,v.8, n° 1, 2023. -- e55.pdf | — | 2.24 MB | Adobe PDF |