Por favor, use este identificador para citar o enlazar este ítem: https://hdl.handle.net/20.500.12008/56155

Título:

5-HT2A Receptor Knockout Mice Show Sex-Dependent Differences following Acute Noribogaine Administration

Otros títulos:

Coordinador:

Director:

Compilador:

Autor:

Villalba, Sofía
González, Bruno
Junge, Stephanie
Bernardi, Alejandra
González, Joaquín
Fagúndez, Catherine
Torterolo, Pablo
Carrera, Ignacio
Urbano, Francisco J.
Bisagno, Verónica

Tutor:

Tipo de documento:

Artículo

Editor:

Palabras clave:

NMDA
Glutamate receptors
Immediate early genes (IEG)
Noribogaine
Psychedelics
Serotonin

Descriptores:

ANIMALES
RATONES
RATONES NOQUEADOS
ÁCIDO GLUTÁMICO
IBOGAÍNA
MEDICAMENTOS SIMILARES
FARMACOLOGÍA
N-METILASPARTATO
ARN MENSAJERO
GENÉTICA
RECEPTOR DE SEROTONINA 5-HT2A
SEROTONINA

Año de publicación:

2024

Contenido:

Resumen:

Noribogaine (noribo) is the primary metabolite from ibogaine, an atypical psychedelic alkaloid isolated from the root bark of the African shrub Tabernanthe iboga. The main objective of this study was to test the hypothesis that molecular, electrophysiological, and behavioral responses of noribo are mediated by the 5-HT2A receptor (5-HT2AR) in mice. In that regard, we used male and female, 5-HT2AR knockout (KO) and wild type (WT) mice injected with a single noribo dose (10 or 40 mg/kg; i.p.). After 30 min., locomotor activity was recorded followed by mRNA measurements by qPCR (immediate early genes; IEG, glutamate receptors, and 5-HT2AR levels) and electrophysiology recordings of layer V pyramidal neurons from the medial prefrontal cortex. Noribo 40 decreased locomotion in male, but not female WT. Sex and genotype differences were observed for IEG and glutamate receptor expression. Expression of 5-HT2AR mRNA increased in the mPFC of WT mice following Noribo 10 (males) or Noribo 40 (females). Patch-clamp recordings showed that Noribo 40 reduced the NMDA-mediated postsynaptic current density in mPFC pyramidal neurons only in male WT mice, but no effects were found for either KO males or females. Our results highlight that noribo produces sexually dimorphic effects while the genetic removal of 5HT2AR blunted noribo-mediated responses to NMDA synaptic transmission.

Descripción:

metadata.articulos.dc.description.uri:

Editorial:

MDPI

EN:

International Journal of Molecular Sciences. 2024;25(2)

Financiadores:

Citación:

Villalba S, González B, Junge S y otros. 5-HT2A Receptor Knockout Mice Show Sex-Dependent Differences following Acute Noribogaine Administration. International Journal of Molecular Sciences [en línea]. 2024;25(2). 18 p.

Citación:

metadata.articulos.cc.license.name:

ISBN:

e-ISBN:

ISSN:

ISMN:

Otros identificadores:

Cobertura geográfica:

Cobertura temporal:

Departamento académico:

Grupo de investigación:

Licencia:

Licencia Creative Commons Atribución (CC - By 4.0)

Colecciones:

Publicaciones Académicas y Científicas
FicherosDescripciónTamañoFormato
5 HT2A Receptor Knockout Mice Show Sex Dependent Differences.pdf 5 HT2A Receptor Knockout Mice Show Sex Dependent Differences. 8.61 MB Adobe PDF
Mostrar el registro Dublin Core completo del ítem