Título:
A confocal microscopy image analysis method to measure adhesion and internalization of Pseudomonas aeruginosa multicellular structures into epithelial cells
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Autor:
Lepanto, Paola
Lecumberry, Federico
Rossello, Jéssica
Kierbel, Arlinet
Lecumberry, Federico
Rossello, Jéssica
Kierbel, Arlinet
Tutor:
Tipo de documento:
Postprint
Editor:
Palabras clave:
Pseudomonas aeruginosa
Adhesion
lnternalization
Multicellular structures
lmage analysis
Adhesion
lnternalization
Multicellular structures
lmage analysis
Descriptores:
Año de publicación:
2014
Contenido:
Resumen:
Formation of multicellular structures such as biofilms is an important feature in the physiopathology of many disease-causing bacteria. We recently reported that Pseudomonas aeruginosa adheres to epithelial cells rapidly forming early biofilm-like aggregates, which can then be internalized into cells. Conventional methods to measure adhesion/internalization, such as dilution plating for total cell-associated or antibiotic protected bacteria, do not distinguish between single and aggregated bacteria. We report a procedure that combining double bacteria labeling, confocal microscopy and image analysis allows identification and quantification of the number of adhered and internalized bacteria distinguishing between single and aggregated bacterial cells. A plugin for Fiji to automatically perform these procedures has been generated.
Descripción:
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EN:
Molecular and Cellular Probes, v, 28, no. 1, 2014.
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Citación:
Lepanto, P, Lecumberry, F, Rossello, J, y otros. "A confocal microscopy image analysis method to measure adhesion and internalization of Pseudomonas aeruginosa multicellular structures into epithelial cells" Molecular and Cellular Probes, v. 28, no. 1, 2014. https://doi.org/10.1016/j.mcp.2013.10.001
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Departamento académico:
Procesamiento de Señales
Grupo de investigación:
Tratamiento de Imágenes
Licencia:
Licencia Creative Commons Atribución - No Comercial - Sin Derivadas (CC - By-NC-ND 4.0)
Colecciones:
| Ficheros | Descripción | Tamaño | Formato | ||
|---|---|---|---|---|---|
| LLRK2014.pdf | — | 582.28 KB | Adobe PDF |